Objective:Natriuretic peptides participate in the collection of metabolic effects during alcohol withdrawal. Having witnessed modulation of other natriuretic peptides in alcohol-dependent patients during alcohol withdrawal, we were interested in the relation of BNP methylation with protein expression and craving in this longitudinal study.
Methods: 99 male patients were compared to 101 healthy controls concerning epigenetic regulation and protein expression during detoxification treatment.
Results: With BNP expression being GATA4-dependent, we observed a correlation of GATA4 binding site methylation and protein expression. BNP serum levels and OCDS scores are significantly decreased during withdrawal. Focusing on the two CpGs that are between GATA transcription factor binding sites, statistical analysis reveals a reversely proportional methylation pattern, significantly increasing with ongoing detoxification and thereby supporting the observed serum level changes.
Conclusion: Without the functional knowledge about regulation of BNP expression via the GATA transcription factor, it would have been easy to take the mean results of the global CpG data and propose a direct relationship between methylation and expression. Thus, these findings are a voice for functionally and mechanistically approved results.